Título del artículo:

Resistance to beta-lactam antibiotics and quinolones in Escherichia coli isolated from broilers

The aim of this study was to determine the antimicrobial resistance of 176 Escherichia coli strains isolated from broiler chicken organs. The strains were challenged with beta-lactam, quinolones and fluoroquinolones, observing resistance to beta-lactam antibiotics (97.7%) and quinolones (86.7%). The results showed that 71.6% of the isolates phenotypically expressed the production of extended spectrum beta-lactamases (ESLB). By PCR, resistance genes for beta-lactams blaTEM, blaSHV, blaCTX-M1 and Amp-C and resistance genes for quinolones qnrA, qnrB, qnrS were determined. The genes AmpC (74%), blaCTX-M (65%), blaSHV (65%), blaTEM (50%), qnrB (86.4%) and qnrS (11.9%) were found. The qnrA gene was not evident in the samples analysed. The results obtained revealed a large percentage of resistance to the studied antibiotics and the presence of resistance genes in isolates from poultry for human consumption, which constitutes a risk for Public Health.

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Revista de Investigaciones Veterinarias del Peru

Título del artículo:

Effect of ibuprofen on the establishment of the gestation of in vitro produced bovine embryos

The aim of this study was to evaluate the effect of the administration of ibuprofen in the establishment of the gestation of bovine embryos produced in vitro. In total, 85 Bos taurus x B. indicus heifers were used as embryo recipients, which were subjected to a protocol of synchronization of oestrus and ovulation with the insertion of an intravaginal progesterone implant (Sincrogest®) for eight days, plus the application of 2 mg of oestradiol benzoate (Sincrodiol®). To the removal of the implant, 500 µg sodium cloprostenol (Sincrocio®), 300 IU of equine chorionic gonadotropin (Sincro eCG®), plus the administration of 1 mg of oestradiol cypionate (SincroCP®,) were applied. One hour before embryo transfer, 5 mg/kg of ibuprofen IM was applied. No significant difference was observed in the gestation rate of the control group (47.5%) and the ibuprofen group (35.5%). It is concluded that the administration of ibuprofen in B. taurus x B. indicus heifers one hour before the transfer of the embryos in vitro had no effect on the establishment of gestation.

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Revista de Investigaciones Veterinarias del Peru

Título del artículo:

Reliability of the scale of barriers for cardiac rehabilitation in the colombian population

Cardiac rehabilitation is supported by the highest level of scientific evidence. However, less than 25% of those eligible to participate in a cardiac rehabilitation program initiate it; and of these, 50% drop out prematurely. A modified Spanish Cardiac Rehabilitation Barriers Scale (CRBS) has been translated, culturally adapted and validated in Colombia, however, the reliability remains to be evaluated. This study aimed to determine the internal consistency and test–retest reliability of the CRBS in a Colombian population. In total, 193 patients (67% men, average age = 65 ± 12 years) completed the scale twice, with an average of eight days between applications. Cronbach’s Alpha and intraclass correlation coefficients (ICC) were calculated. The internal consistency of the Colombian version of the CRBS was acceptable (Cronbach’s alpha = 0.84). The ICC of the CRBS was 0.69 (95% CI 0.61–0.76); 0.78 (95% CI 0.71–0.84) when the CRBS was completed by interview; and 0.47 (95% CI 0.21–0.67) when the CRBS was self-reported. The reliability of the interview version of the CRBS was substantial in the Colombian population; however, the reliability of the self-report version was lower. The use of this scale will allow developing strategies to increase participation and adherence to cardiac rehabilitation programs.

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International Journal of Environmental Research and Public Health

Título del artículo:

Genotypic and functional characterization of mutations in the reverse transcriptase domain of the hepatitis B virus P gene in patients coinfected with human immunodeficiency virus receiving antiretrovirals

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Iatreia

Título del artículo:

Determination of molecular markers for hepatitis B through deep sequencing of the viral genome and the miRNA expression in samples obtained from blood banks in Colombia

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Iatreia

Título del artículo:

Development of an aptamer-based electrochemical biosensor for the detection of PSA and its isoform p2PSA

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Iatreia

Título del artículo:

Clinical characteristics and findings on computed tomography of the thorax in patients with COVID-19

The pandemic generated by COVID-19 has generated an enormous medical and socioeconomic cost, which has led the scientific field and the different medical associations to make all their resources available to find a prompt solution to face this pathology. The objective of this review is to describe the most relevant clinical characteristics and chest computed tomography findings in patients with COVID-19; By identifying scientific articles in databases. Fifteen studies were included that met the inclusion criteria defined by the authors. The most relevant findings show us that SARS-CoV-2 has a rapid and generalized transmission, with the involvement of multiple systems, but with a predominance of the respiratory tract. Most patients have mild symptoms; the main manifestations are fever, cough, shortness of breath, muscle pain, headache, etc. Patients with more severe diseases and requiring admission to the ICU are older and have more comorbidity compared to the general population. The current recommendations of the American College of Radiology for the use of chest CT, we propose to reserve it for hospitalized patients when it is necessary to determine their treatment. In conclusion, due to the great variety of clinical manifestations and the involvement of multiple organs, there are no specific clinical characteristics that allow distinguishing COVID-19 from other respiratory infections of viral etiology.

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Gaceta Medica de Caracas

Título del artículo:

Urinary Sodium and Potassium, and Risk of Ischemic and Hemorrhagic Stroke (INTERSTROKE): A Case-Control Study

Although low sodium intake (<2 g/day) and high potassium intake (>3.5 g/day) are proposed as public health interventions to reduce stroke risk, there is uncertainty about the benefit and feasibility of this combined recommendation on prevention of stroke. METHODS: We obtained random urine samples from 9,275 cases of acute first stroke and 9,726 matched controls from 27 countries and estimated the 24-hour sodium and potassium excretion, a surrogate for intake, using the Tanaka formula. Using multivariable conditional logistic regression, we determined the associations of estimated 24-hour urinary sodium and potassium excretion with stroke and its subtypes. RESULTS: Compared with an estimated urinary sodium excretion of 2.8-3.5 g/day (reference), higher (>4.26 g/day) (odds ratio [OR] 1.81; 95% confidence interval [CI], 1.65-2.00) and lower (<2.8 g/day) sodium excretion (OR 1.39; 95% CI, 1.26-1.53) were significantly associated with increased risk of stroke. The stroke risk associated with the highest quartile of sodium intake (sodium excretion >4.26 g/day) was significantly greater (P < 0.001) for intracerebral hemorrhage (ICH) (OR 2.38; 95% CI, 1.93-2.92) than for ischemic stroke (OR 1.67; 95% CI, 1.50-1.87). Urinary potassium was inversely and linearly associated with risk of stroke, and stronger for ischemic stroke than ICH (P = 0.026). In an analysis of combined sodium and potassium excretion, the combination of high potassium intake (>1.58 g/day) and moderate sodium intake (2.8-3.5 g/day) was associated with the lowest risk of stroke. CONCLUSIONS: The association of sodium intake and stroke is J-shaped, with high sodium intake a stronger risk factor for ICH than ischemic stroke. Our data suggest that moderate sodium intake - rather than low sodium intake - combined with high potassium intake may be associated with the lowest risk of stroke and expected to be a more feasible combined dietary target.

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American Journal of Hypertension

Título del artículo:

Comparison between five coprological methods for the diagnosis of Balantidium coli cysts in fecal samples from pigs

Background and Aim: Balantidium coli is a protozoan that can infect humans and non-human primates, being the domestic pigs the animals most affected by this parasite. This study aimed to compare the performance of five coprological methods for diagnosis of Balantidium coli cysts in fecal samples from pigs. Materials and Methods: From September to December 2019, 558 fecal samples were collected from the rectum of backyard pigs in the Bucaramanga metropolitan area, Colombia. The average age of the sampled animals was 3-4 months. Fecal samples collected were tested using the following coprological techniques: Direct examination with Lugol\'s iodine solution, buffered saline, centrifugation/flotation and McMaster techniques, and Ziehl-Neelsen method. Results: The results indicate that B. coli cysts were diagnosed most frequently through direct examination with Lugol\'s iodine solution (52.7% of the samples) followed by direct examination with a buffered saline solution (37.6%). Moderate concordance (k=0.41; p<0.05) was determined between direct method with Lugol\'s iodine and buffered saline solution, McMaster and buffered saline (k=0.35; p<0.05), and centrifugation/flotation and buffered saline (k=0.28; p<0.05) showed a fair degree of concordance. The rest of the comparisons were classified as poor. The flotation techniques (centrifugation/ flotation and McMaster) did not show good recovery of cysts. Conclusion: It is concluded that the most efficient method for diagnosing this parasitosis was to an association between direct examination with Lugol\'s iodine solution and buffered saline.

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Veterinary World

Título del artículo:

Rabies and the Heart

As one of the neglected diseases, rabies is as a highly fatal viral infection, most prevalent in low-and middle-income regions, which produces a substantial health and economic burden. It mainly affects the central nervous system causing encephalitis, however extraneuronal involvement has been documented. Cardiac structures may be involved and can play a role in the severity of the disease. Most of the existing literature comes from case reports and case series where cardiac involvement results in myocarditis and cardiac arrhythmias. As part of the “Neglected Tropical Diseases and Other Infectious Diseases Involving the Heart” (NET-HEART Project), the objective of this article is to review all the information available on the cardiac involvement of this disease.

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Cardiology Research

Título del artículo:

The SIMAC study: A randomized controlled trial to compare the effects of resistance training and aerobic training on the fitness and body composition of Colombian adolescents

The aim of this study was to evaluate the impact on muscle strength, aerobic fitness and body composition, of replacing the physical education (PE) class of Colombian adolescents with resistance or aerobic training. 120 tanner stage 3 adolescents attending a state school were randomized to resistance training, aerobic training, or a control group who continued to attend a weekly 2- hour PE class for 16 weeks. The resistance training and aerobic training groups participated in twice weekly supervised after-school exercise sessions of 1 hour instead of their PE class. Sum of skinfolds, lean body mass (bioelectrical impedance analysis), muscular strength (6 repetition maximum (RM)) bench press, lateral pulldown and leg press) and estimated cardiorespiratory fitness (multistage 20 meter shuttle run) were ssessed at pre and post intervention. Complete data were available for n = 40 of the resistance training group, n = 40 of the aerobic training group and n = 30 PE (controls). Resistance raining attenuated increases in sum of skinfolds compared with controls (d = 0.27, [0.09–0.36]). We found no significant effect on lean body mass. Resistance training produced a positive effect on muscle strength compared with both controls (d = 0.66 [.49-.86]) and aerobic training (d = 0.55[0.28–0.67]). There was a positive effect of resistance training on cardiorespiratory fitness compared with controls (d = 0.04 [-0.10–0.12]) but not compared with aerobic training (d = 0.24 [0.10–0.36]). Replacing a 2-hour PE class with two 1 hour resistance training sessions attenuated gains in subcutaneous adiposity, and enhanced muscle strength and aerobic fitness development in Colombian youth, based on a median attendance of approximately 1 session a week. Further research to assess whether adequate stimuli for the development of muscular fitness exists within current physical education provision is warranted.

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PLoS ONE

Título del artículo:

Environmental risk factors associated with respiratory diseases in children with socioeconomic disadvantage

Children are more vulnerable to environmental exposures determinant of respiratory diseases due to their dynamic developmental physiology. Whereas social determinants of health are also associated with a higher risk of these diseases in children exposed to environmental risk factors, most studies incorporate them as covariates in the statistical analysis rather than focusing on specific vulnerable populations. In this study a systematic review searched and selected studies of respiratory diseases in children with socioeconomic disadvantage to identify the environmental risk factors associated with these diseases. The review followed the PRISMA protocol to identify eleven eligible studies of children with socioeconomic conditions that included low income and low socioeconomic status, overcrowding, adults with low education level and Indigenous status. Infectious respiratory diseases, asthma, rhinitis and mortality due to respiratory diseases were associated with risk factors such as biomass fuel use, tobacco smoking, particulate matter, coal dust and other pollutants including ozone, nitrogen dioxide and sulphur dioxide. The most common associations were between respiratory infections and household air pollution and asthma with indoor and outdoor air pollution. The findings support previous reports on these associations and suggest that specific vulnerabilities such as indigenous children and living with adults with low socioeconomic status and education level increase the risk of respiratory diseases. These populations can be given special attention to prioritize public health interventions to lower the burden of disease of respiratory diseases in children.

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Heliyon

Título del artículo:

Fungal Endophytes of Tahiti Lime (Citrus citrus × latifolia) and Their Potential for Control of Colletotrichum acutatum J. H. Simmonds Causing Anthracnose

Colletotrichum acutatum is one of the causal agents of anthracnose in several crops, and of post-flowering fruit drop (PFD) in citrus and key lime anthracnose (KLA). The pathogen normally attacks flowers, causing lesions only in open flowers. Under very favorable conditions, however, it can also affect flower buds and small fruits, causing complete rotting of the fruit and a premature fall, resulting in major economic crop losses. We isolated endophytic fungi from Tahiti lime to evaluate its diversity, verify its antagonistic capacity against the phytopathogen Colletotrichum acutatum C-100 in dual tests, and evaluate the ability of various endophytic agents to control flowers with induced anthracnose. 138 fungal isolates were obtained from 486 fragments of branches, leaves, and fruit; from which 15 species were identified morphologically. A higher isolation frequency was found in branches and leaves, with a normal level of diversity compared to other citrus species. Of the 15 morphospecies, 5 were trialed against C. acutatum in antagonism tests, resulting in a finding of positive inhibition. 2 endophytic fungi from the antagonism tests demonstrated high inhibition of the phytopathogen, and were thus used in in vivo tests with Tahiti lime flowers, applied in a spore solution. Spore solutions of two molecularly identified species, Xylaria adscendens, and Trichoderma atroviride, reduced the lesions caused by the phytopathogen in these in vivo tests. The finding that these endophytes react antagonistically against C. acutatum may make them good candidates for further biological control research in an agroindustry that requires environmental sustainability.

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Frontiers in Bioengineering and Biotechnology

Título del artículo:

Mapping stages, barriers and facilitators to the implementation of HEARTS in the Americas initiative in 12 countries: A qualitative study

The World Health Organization (WHO) Global Hearts Initiative offers technical packages to reduce the burden of cardiovascular diseases through population-wide and targeted health services interventions. The Pan American Health Organization (PAHO) has led implementation of the HEARTS in the Americas Initiative since 2016. The authors mapped the developmental stages, barriers, and facilitators to implementation among the 371 primary health care centers in the participating 12 countries. The authors used the qualitative method of document review to examine cumulative country reports, technical meeting notes, and reports to regional stakeholders. Common implementation barriers include segmentation of health systems, overcoming health care professionals\' scope of practice legal restrictions, and lack of health information systems limiting operational evaluation and quality improvement mechanisms. Main implementation facilitators include political support from ministries of health and leading scientific societies, PAHO\'s role as a regional catalyst to implementation, stakeholder endorsement demonstrated by incorporating HEARTS into official documents, and having a health system oriented to primary health care. Key lessons include the need for political commitment and cultivating on-the-ground leadership to initiate a shift in hypertension care delivery, accompanied by specific progress in the development of standardized treatment protocols and a set of high-quality medicines. By systematizing an implementation strategy to ease integration of interventions into delivery processes, the program strengthened technical leadership and ensured sustainability. These study findings will aid the regional approach by providing a staged planning model that incorporates lessons learned. A systematic approach to implementation will enhance equity, efficiency, scale-up, and sustainability, and ultimately improve population hypertension control.

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Journal of Clinical Hypertension

Título del artículo:

Association of bedtime with mortality and major cardiovascular events: an analysis of 112,198 individuals from 21 countries in the PURE study

Objectives: This study aimed to examine the association of bedtime with mortality and major cardiovascular events. Methods: Bedtime was recorded based on self-reported habitual time of going to bed in 112,198 participants from 21 countries in the Prospective Urban Rural Epidemiology (PURE) study. Participants were prospectively followed for 9.2 years. We examined the association between bedtime and the composite outcome of all-cause mortality, non-fatal myocardial infarction, stroke and heart failure. Participants with a usual bedtime earlier than 10PM were categorized as ‘earlier’ sleepers and those who reported a bedtime after midnight as ‘later’ sleepers. Cox frailty models were applied with random intercepts to account for the clustering within centers. Results: A total of 5633 deaths and 5346 major cardiovascular events were reported. A U-shaped association was observed between bedtime and the composite outcome. Using those going to bed between 10PM and midnight as the reference group, after adjustment for age and sex, both earlier and later sleepers had a higher risk of the composite outcome (HR of 1.29 [1.22, 1.35] and 1.11 [1.03, 1.20], respectively). In the fully adjusted model where demographic factors, lifestyle behaviors (including total sleep duration) and history of diseases were included, results were greatly attenuated, but the estimates indicated modestly higher risks in both earlier (HR of 1.09 [1.03–1.16]) and later sleepers (HR of 1.10 [1.02–1.20]). Conclusion: Early (10 PM or earlier) or late (Midnight or later) bedtimes may be an indicator or risk factor of adverse health outcomes.

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Sleep Medicine

Título del artículo:

A proposal for the transformation of fossil fuel energy economies to hydrogen economies through social entrepreneurship

The world is growing at an ever-increasing rate, but this growth has occurred alongside an energy model based onfossilfuels, which presents serious sustainability problems. The current study seeks to illustrate one of the new alternative energy schemes which could replace fossil fuels in the future: Hydrogen. The promise of hydrogen fuel has led large nations to invest in transitioning their fossil fuel economies to a hydrogen-based model. This will result in significant social impacts, leading to the question, How should it be done? Social entrepreneurship can integrate socio-economic actors and agents and help them to devise and implement new forms of energy innovation, which will open spaces for new business models. The current study conceptualizes within a present-day context the connotations of the hydrogen economy at an international level, its implications to society, and its synergy with social entrepreneurship. The authors observed the rapid growth that these entrepreneurship initiatives are generating and how the models used to manage these undertakings play a vital role.

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Entrepreneurial Innovation for Securing Long-Term Growth in a Short-Term Economy

Título del artículo:

Phytoremediation of heavy metals in tropical soils an overview

The geomorphological characteristics of the materials inherent in tropical soils, in addition to the excessive use of fertilizers and pesticides, industrial waste and residues, and novel pollutants derived from emerging new technologies such as nanomaterials, affect the functionality and resilience of the soil-microorganism-plant ecosystem; impacting phytoremediation processes and increasing the risk of heavy metal transfer into the food chain. The aim of this review is to provide a general overview of phytoremediation in tropical soils, placing special emphasis on the factors that affect this process, such as nanoagrochemicals, and highlighting the value of biodiversity among plant species that have the potential to grow and develop in soils impacted by heavy metals, as a useful resource upon which to base further research.

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Sustainability (Switzerland)

Título del artículo:

Analysis of the Formal/Informal Wage Inequalities in Colombia: A Semiparametric Approach

Informality is a common problem in Colombia, with almost 50 percent of the workers employed in this sector. This may be a solution for unemployment, but it is a lose/lose game unless the individuals have a comparative advantage in the informal sector. This article uses information from the Colombian Great Integrated Household Survey (GIHS) to analyze the wage gap between formal and informal urban sectors in two different periods, 2008:4 and 2017:4, using a semiparametric approach. Kernel density functions by groups are estimated; counterfactuals are generated by weighting wages of informal sector workers by their probability of working in the formal sector, to estimate how much an informal sector worker could make if treated as formal, according to his characteristics. The results indicate that only some groups (self-employed and some entrepreneurs) are better off if formalized.

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Journal of Applied Social Science

Título del artículo:

Rivaroxaban Plus Aspirin in Obese and Overweight Patients With Vascular Disease in the COMPASS Trial

Background: Direct oral anticoagulants are administered in fixed doses irrespective of body weight, but guidelines recommend against their use in patients with extremes of body weight. Objectives: This study determined the effects of dual-pathway inhibition antithrombotic regimen (rivaroxaban 2.5 mg twice daily plus aspirin 100 mg/day) compared with aspirin Halone across a range of patient body mass indexes (BMIs) and body weights. Methods: This was a secondary analysis of the COMPASS (Cardiovascular OutcoMes for People using Anticoagulation StrategieS) trial, which included patients with chronic coronary artery disease or peripheral artery disease. Efficacy and safety outcomes were studied in relation to BMI: (normal 18.5 ≤BMI <25 kg/m2, overweight 25 ≤BMI <30 kg/m2, obese ≥30 kg/m2) and body weight (≤70 kg, 70 < weight ≤90 kg, and >90 kg; as well as ≤120 kg vs. >120 kg). Results: Among 27,395 randomized patients, 6,459 (24%) had normal BMI, 12,047 (44%) were overweight, and 8,701 (32%) were obese. The combination of rivaroxaban and aspirin compared with aspirin produced a consistent reduction in the primary outcome of cardiovascular death, stroke, or myocardial infarction, irrespective of BMI or body weight. For 18.5 ≤BMI <25 kg/m2: 3.5% vs. 5.0%; hazard ratio (HR): 0.73 (95% credible interval [CrI]: 0.58 to 0.90); 25 ≤ BMI <30 kg/m2: 4.3% vs. 5.1%; HR: 0.80 (95% CrI: 0.66 to 0.96); BMI ≥30 kg/m2: 4.2% vs. 6.1%; HR: 0.71 (95% CrI: 0.57 to 0.86). For body weight ≤70 kg: 4.1% vs. 5.3%; HR: 0.75 (95% CrI: 0.62 to 0.91); 70 < weight ≤90 kg: 4.1% vs. 5.3%; HR: 0.76 (95% CrI: 0.65 to 0.89); >90 kg: 4.2% vs. 5.7%; HR: 0.74 (95% CrI: 0.61 to 0.90). Effects on bleeding, mortality, and net clinical benefit were consistent irrespective of BMI or bodyweight. Conclusions: The effects of dual-pathway antithrombotic therapy are consistent irrespective of BMI or body weight, suggesting no need for dose adjustments in the ranges of weights and BMI of patients enrolled in the COMPASS trial. Further studies need to address this problem in relation to greater extremes of body weight. (Rivaroxaban for the Prevention of Major Cardiovascular Events in Coronary or Peripheral Artery Disease [COMPASS]; NCT01776424)

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Journal of the American College of Cardiology

Título del artículo:

Associations of cereal grains intake with cardiovascular disease and mortality across 21 countries in prospective urban and rural epidemiology study: Prospective cohort study

Objective To evaluate the association between intakes of refined grains, whole grains, and white rice with cardiovascular disease, total mortality, blood lipids, and blood pressure in the Prospective Urban and Rural Epidemiology (PURE) study. Design Prospective cohort study. setting PURE study in 21 countries. ParticiPants 148 858 participants with median follow-up of 9.5 years. exPOsures Country specific validated food frequency questionnaires were used to assess intakes of refined grains, whole grains, and white rice. Main OutcOMe Measure Composite of mortality or major cardiovascular events (defined as death from cardiovascular causes, nonfatal myocardial infarction, stroke, or heart failure). Hazard ratios were estimated for associations of grain intakes with mortality, major cardiovascular events, and their composite by using multivariable Cox frailty models with random intercepts to account for clustering by centre. results Analyses were based on 137 130 participants after exclusion of those with baseline cardiovascular disease. During follow-up, 9.2% (n=12 668) of these participants had a composite outcome event. The highest category of intake of refined grains (≥350 g/ day or about 7 servings/day) was associated with higher risk of total mortality (hazard ratio 1.27, 95% confidence interval 1.11 to 1.46; P for trend=0.004), major cardiovascular disease events (1.33, 1.16 to 1.52; P for trend<0.001), and their composite (1.28, 1.15 to 1.42; P for trend<0.001) compared with the lowest category of intake (<50 g/day). Higher intakes of refined grains were associated with higher systolic blood pressure. No significant associations were found between intakes of whole grains or white rice and health outcomes. cOnclusiOn High intake of refined grains was associated with higher risk of mortality and major cardiovascular disease events. Globally, lower consumption of refined grains should be considered.

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The BMJ

Título del artículo:

Marine Seagrass Extract of Thalassia testudinum Suppresses Colorectal Tumor Growth, Motility and Angiogenesis by Autophagic Stress and Immunogenic Cell Death Pathways

Marine plants have become an inexhaustible reservoir of new phytopharmaceuticals for cancer treatment. We demonstrate in vitro/in vivo antitumor efficacy of a standardized polyphenol extract from the marine angiosperm Thalassia testudinum (TTE) in colon tumor cell lines (RKO, SW480, and CT26) and a syngeneic allograft murine colorectal cancer model. MTT assays revealed a dosedependent decrease of cell viability of RKO, CT26, and SW480 cells upon TTE treatment with IC50 values of, respectively, 175, 115, and 60 µg/mL. Furthermore, TTE significantly prevented basal and bFGF-induced angiogenesis in the chicken chorioallantoic membrane angiogenesis assay. In addition, TTE suppressed bFGF-induced migration of endothelial cells in a wound closure assay. Finally, TTE treatment abrogated CT26 colorectal cancer growth and increased overall organism survival in a syngeneic murine allograft model. Corresponding transcriptome profiling and pathway analysis allowed for the identification of the mechanism of action for the antitumor effects of TTE. In line with our in vitro/in vivo results, TTE treatment triggers ATF4-P53-NFκB specific gene expression and autophagy stress pathways. This results in suppression of colon cancer cell growth, cell motility, and angiogenesis pathways in vitro and in addition promotes antitumor immunogenic cell death in vivo.

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Marine Drugs

Título del artículo:

Assessment of ambivalent sexism in university students in colombia and spain: A comparative analysis

(1) Background: Gender-based violence has no geographical, personal, or social boundaries. It constitutes a serious public health problem that affects the entire society. This research aims to identify and compare the level of ambivalent sexism in Spanish and Colombian university students and its relationship with sociodemographic factors. Ambivalent sexism, developed by Glick and Fiske (1996), is considered a new type of sexism since, for the first time, it combines negative and positive feelings that give rise to hostile and benevolent sexism, maintaining the subordination of women through punishment and rewards. (2) Methods: The methodology consisted of the application of the validated Spanish version of the Ambivalent Sexism Inventory (ASI) to a sample of 374 students in their final academic year of the Law program, of which 21.7% were students at the University of Santander (Bucaramanga, Colombia), 45.5% at the University Rey Juan Carlos (Madrid, Spain), and the remaining 32.9% at the University of Seville (Seville, Spain). (3) Results: A high level of ambivalent sexism is reported in Colombian students nowadays. In the two countries. there are similarities (e.g., the great weight of religion and the variation in attitudes towards sexism in people who identify themselves as women, compared to male or students consulted that prefer not to answer) and differences (e.g., absence in Colombia of gender-specific legislation, low number of students who have received gender education in Spain). (4) Conclusions: These findings may contribute to the construction of laws that take into account the particular problems of women and the development of educational programs on gender that are offered in a transversal and permanent way and that take into account cultural factors and equity between men and women as an essential element in the training of future judges who have the legal responsibility to protect those who report gender violence.

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International Journal of Environmental Research and Public Health

Título del artículo:

Does Muscle–Tendon Unit Structure Predispose to Hamstring Strain Injury During Running? A Critical Review

Hamstring strain injury (HSI) remains the most common muscle injury in high-intensity running in humans. The majority of acute HSI occur specifically within the proximal region of the long head of biceps femoris and there is a sustained interest among researchers in understanding the factors that predispose to HSI. The present critical review describes the current understanding of biceps femoris long head (BFlh) structural features that might influence strain injury risk. Inter-individual differences in muscle–tendon architecture and interactions, muscle fiber type and region-specific innervation are likely to influence biceps femoris long head injury risk and might inform why some individuals are at an increased risk of sustaining a HSI during running. However, more research is needed, with future studies focusing on prospective data acquisition, improved computer simulations and direct imaging techniques to better understand the relationship between structural features, hamstring muscle function, and injury risk.

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Sports Medicine

Título del artículo:

Orientation of Belminus triatomines to cockroaches and cockroaches’ fecal volatiles: an ethological approach

Most triatomine bugs (Hemiptera: Reduviidae: Triatominae) are hematophagous, though Belminus species can live off of cockroach hemolymph to complete their life cycle. In this work, we described the fixed action pattern (FAP) employed by B. ferroae to identify, approach, and suck on a living cockroach. The FAP described here is composed of the following stereotyped behaviors: (1) visual and/or olfactory detection of the cockroach, (2) reaching, (3) cautious approach, (4) antennal exploration, (5) extension of the proboscis, (6) piercing to sedate, (7) walking away and waiting (postsedation behavior), (8) second cautious approach, (9) extension of the proboscis, (10) piercing to suck hemolymph. The FAP sequence observed suggests that Belminus bugs are not predators like the rest of reduviids (assassin bugs)—but are kleptophagous ectoparasites, since they do not attack and kill a prey but rather steal hemolymph from its invertebrate host. Based on these ethological observations, we propose kleptophagy as a trait that naturally groups the Belminus species into the Triatominae subfamily. In order to identify chemicals cues that could elicit such FAP, we examined the behavior of B. corredori, B. ferroae, and B. herreri in response to the cockroaches’ odor, fresh cockroach feces and fresh rodent wastes. The last two sources were tested based on the assumption that abundant chemicals near host refuges could serve as cues for host orientation. We found that the cockroach odor emanating from a box significantly attracted B. corredori and B. herreri in a still air olfactometer. The three Belminus species approached the captive cockroach after 1 h, but avoided to climb the box. Odors emanating from the cockroach feces attracted B. corredori and B. ferroae in a Y-olfactometer. Triatomines and their hosts have intimately shared the same refuge for millions of years; certain molecules occur across invertebrate and vertebrate refuges and are recurrent in human abodes, thus plausibly explaining how these bugs can readily switch to the domestic habitat, while keeping with their kleptophagous nature.

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Acta Ethologica

Título del artículo:

Spatial clustering of orientation preference in primary visual cortex of the large rodent agouti

All rodents investigated so far possess orientation-selective neurons in the primary visual cortex (V1) but – in contrast to carnivores and primates – no evidence of periodic maps with pinwheel-like structures. Theoretical studies debating whether phylogeny or universal principles determine development of pinwheels point to V1 size as a critical constraint. Thus, we set out to study maps of agouti, a big diurnal rodent with a V1 size comparable to cats\'. In electrophysiology, we detected interspersed orientation and direction-selective neurons with a bias for horizontal contours, corroborated by homogeneous activation in optical imaging. Compatible with spatial clustering at short distance, nearby neurons tended to exhibit similar orientation preference. Our results argue against V1 size as a key parameter in determining the presence of periodic orientation maps. They are consistent with a phylogenetic influence on the map layout and development, potentially reflecting distinct retinal traits or interspecies differences in cortical circuitry.

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